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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was located to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two more splice variants have been reported but in-depth nucleotide sequence data is not still obtainable.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope identified through the antibody.
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This guideline outlines even further possible optimization of cellular and immunoassay parameters to be certain the best possible results are...
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This information outlines further more achievable optimization of cellular and immunoassay parameters to be certain the best possible final results are...
Antibodies sure to the blocking peptide now not bind to the epitope on the goal protein. By comparing the staining with the blocked antibody compared to the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which lead for the temporal coordination of each and every mitotic celebration. Cyclin E kinds a posh with and functions to be a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates in the G1-S period boundary and is also degraded as cells development by means of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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